ABSTRACT
Background: The aim of this work was to purify and characterize exo-ß-1,3-glucanase, namely, TtBgnA, from the thermophilic fungus Thielavia terrestris Co3Bag1 and to identify the purified enzyme. Results: The thermophilic biomass-degrading fungus T. terrestris Co3Bag1 displayed ß-1,3-glucanase activity when grown on 1% glucose. An exo-ß-1,3-glucanase, with an estimated molecular mass of 129 kDa, named TtBgnA, was purified from culture filtrates from T. terrestris Co3Bag1. The enzyme exhibited optimum activity at pH 6.0 and 70°C and half-lives (t1/2) of 54 and 37 min at 50 and 60°C, respectively. Substrate specificity analysis showed that laminarin was the best substrate studied for TtBgnA. When laminarin was used as the substrate, the apparent KM and Vmax values were determined to be 2.2 mg mL-1 and 10.8 U/mg, respectively. Analysis of hydrolysis products by thin-layer chromatography (TLC) revealed that TtBgnA displays an exo mode of action. Additionally, the enzyme was partially sequenced by tandem mass spectrometry (MS/MS), and the results suggested that TtBgnA from T. terrestris Co3Bag1 could be classified as a member of the GH-31 family. Conclusions: This report thus describes the purification and characterization of TtBgnA, a novel exo-ß-1,3-glucanase of the GH-31 family from the thermophilic fungus T. terrestris Co3Bag1. Based on the biochemical properties displayed by TtBgnA, the enzyme could be considered as a candidate for potential biotechnological applications.
Subject(s)
Sordariales/enzymology , Glucan 1,3-beta-Glucosidase/chemistry , Temperature , Enzyme Stability , Cellulases , Glucan 1,3-beta-Glucosidase/isolation & purification , Electrophoresis, Polyacrylamide Gel , Tandem Mass Spectrometry , Enzyme Assays , Hydrogen-Ion ConcentrationABSTRACT
Abstract The aim of this study is to evaluate the Nicaraguan screening program for congenital hypothyroidism in terms of coverage and effectiveness of detection and confirmation of cases with the condition throughout a decade. Thyrotropin was quantified in cord-blood samples by a validated ELISA and a cut-off of 20 mU/l was applied. Coverage, positive predictive value, recall rate and prevalence were retrospectively analysed. Babies with positive screening results were contacted for confirmation by means of determination of thyrotropin and thyroid profile in serum samples. 272,338 babies were screened during the period 2005-2015. The mean coverage reached by the program in the participating departments was 71%, with a positive predictive value of 83% and a recall rate of 0.055%. Eighty cases of congenital hypothyroidism were identified, representing an incidence of 1 in 3229 live births, most of them (81%) being severe. The performance of the Nicaraguan screening program is comparable to those in Latin America also using cord-blood samples. The incidence of congenital hypothyroidism is within the low range of other countries worldwide. Strategies are needed to expand the program to the whole country, improve recall rates and achieve earlier treatment of babies, with the condition.
ABSTRACT
En 1957, L. D. Erasmus comunicó la asociación entre sílice y esclerosis sistémica, destacando la importancia de la exposición a sílice como un factor de riesgo para el desarrollo de esclerodermia ocupacional. Si bien existen reportes de la interacción entre sílice y el sistema inmune, continúa siendo actualmente una asociación infrecuente. El objetivo es presentar un paciente varón de 41 años con esclerosis sistémica e historia de exposición a gran cantidad de polvo de sílice, que desarrolló síndrome de Erasmus. Realizamos además una revisión de la literatura.
In 1957, L. D. Erasmus reported the association between silica andsystemic sclerosis, highlighting the importance of the silica exposureas a risk factor for developing scleroderma occupational. While thereare reports of the interaction between silica and the immune systemcurrently remains an infrequent association.The objective is to report a 41 year old male patient with systemicsclerosis and history of high quantity of silica dust exposure whodeveloped an Erasmus syndrome. A review of the literature has alsobeen performed.
Subject(s)
Scleroderma, Systemic , SilicosisABSTRACT
Pregunta de investigación. ¿Cual sera la validez del índice de masa corporal en embarazadas con relación al nomograma de Rosso y Mardones en los hospitales materno infantil, de la mujer y boliviano holandés de la ciudad de La Paz de enero a febrero gestión 2002?. Objetivo general. Determinar la sensibilidad, especificidad, valores predictivos positivos y negativo, además de los likelihood ratio del índice de masa corporal en embarazadas con relación al nomograma de Pedro Rosso y Mardones en los hospitales materno infantil, de la mujer y boliviano holandés de enero a febrero gestíon 2002. Diseño de estudio en la investigación. Test diagnóstico. Lugar. Hospital Materno Infantil, Hospital de la Mujer de la ciudad de La Paz y Hospital Boliviano Holandés de la Ciudad de El Alto. Población. Embarazadas, desde la décima semana a la semana 40 de gestación que asistieron al control prenatal de rutina de los hospitales señalados anteriormente. Métodos. Se evaluó el estado nutricional de la embarazada utilizando el Indice de Masa Corporal IMC (prueba a validarse) y el Nomograma de Rosso y Mardones (Gold Estandar). Para los cálculos de sensibilidad, especificidad, valores predictivos positivos y valores predictivos negativos se utilizó el paquete estadístico EPIDATA y para los cálculos estadísticos el paquete estadístico STATA 6.0. Se realizó el análisis descriptivo utilizando medidas de tendencia central y de dispersión como Promedio, Desviación Estandar, verificando a través del cálculo del intervalo de confianza 95 por ciento, porcentajes y frecuencia para las variables cualitativas. Resultados. El presente estudio se realizó en 479 embarazadas que asistieron al control prenatal de rutina en los meses de enero y febrero del 2002...
What is the validity of the Body Mass Index in pregnant women in relation to the Normogram of Rosso and Mardones in the Hospitals "Materno Infantil" " de la Mujer", and "Boliviano Holandés" in La Paz, from January to February 2002 ? General Objective o determine sensibility, specificity, positive and negative predictive values, in addition to likelihood ratio and Body Mass Index in pregnant women in relation to the Normogram of Pedro Rosso and Mardones in the Hospitals "Materno Infantil", "de la Mujer", and Boliviano Holandés" from January to February 2002. Research Design Diagnostic test SITE"Hospital Materno Infantil", "Hospital de la Mujer", "Hospital Boliviano Holandés" in La Paz. SAMPLE The sample consisted of 479 pregnant women, from the 10th week of gestation to 40 weeks of gestational age who came for routine prenatal control to the above mentioned hospitals. Methods We assessed the nutritional status of the pregnant woman using the Body Mass Index (the test to be validated), and the Normogram of Rosso and Mardones (Gold Standard).For the calculation of sensibility, specificity, negative and positive predictive values, we used the statistic package EPIDATA, and for the statistic calculus the statistic package STATA 6.0.For the descriptive analysis we used measures of central tendency and of dispersion like Mean, Standard Deviation, verifying by means of calculating the Confidence Interval 95%, percentages and frequency for the qualitative values. In order to establish the relationship between both tests we used the tests of Correlation and Chi2 .Result The present study was carried out in 479 pregnant women who came to the routine prenatal control in January and February 2002 in the Hospitals "Materno Infantil", "Boliviano Holandés", and "Hospital de la Mujer".The nutritional diagnose of the preThe results of the...
Subject(s)
Humans , Female , Pregnancy , Nomograms , Body Mass Index , Bolivia , Sensitivity and Specificity , Diagnostic Techniques and Procedures/standardsABSTRACT
Se ha realizado una investigación retrospectiva aprovechando la información estadistica existente en el hospital del Niño de la ciudad de La Paz. Los resultados muestran una estrecha relación entre desnutrició, enfermedad infecciosa y mortalidad, siendo la probabilidad de morir entre los desnutridos 3 veces mayor que la probabilidad de morir entre los no desnutridos. el curso de las Infeciones Respiratorias Agudas y las gastroenteritis se muestra notoriamente relacionado con la presencia de la desnutrición infantil, en cuyo caso el riesgo de muerte aumenta considerablemente, especialmente en las IRA.